human henle 407 cell line Search Results


human epithelial henle 407 cells  (ATCC)
95
ATCC human epithelial henle 407 cells
Human Epithelial Henle 407 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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henle 407  (ATCC)
99
ATCC henle 407
Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and <t>Henle-407</t> (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.
Henle 407, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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henle 407 - by Bioz Stars, 2026-06
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plasmid pcvd442  (ATCC)
99
ATCC plasmid pcvd442
Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and <t>Henle-407</t> (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.
Plasmid Pcvd442, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
plasmid pcvd442 - by Bioz Stars, 2026-06
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epithelial cell line  (ATCC)
96
ATCC epithelial cell line
Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and <t>Henle-407</t> (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.
Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epithelial cell line - by Bioz Stars, 2026-06
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henle-407  (National Centre for Cell Science)
90
National Centre for Cell Science henle-407
Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and <t>Henle-407</t> (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.
Henle 407, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
henle-407 - by Bioz Stars, 2026-06
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Image Search Results


Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.

Journal:

Article Title: Coordinate Intracellular Expression of Salmonella Genes Induced during Infection

doi:

Figure Lengend Snippet: Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.

Article Snippet: The murine macrophage cell line RAW 264.7 and human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine) (ATCC TIB-71, CCL-23, and CCL-6, respectively) were obtained from the American Type Culture Collection, Rockville, Md., and maintained in minimum essential medium (MEM) supplemented with Earle’s salts, l -glutamine, and 10% heat-inactivated fetal calf serum (FCS) (Life Technologies, Rockville, Md.).

Techniques: Expressing, Cell Culture, Incubation, Standard Deviation